Western Blot - Anti-beta III Tubulin Antibody [ARC0456] (A309019)
Western blot analysis of extracts of various cell lines, using Anti-beta III Tubulin Antibody [ARC0456] (A309019) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 3min.
Immunofluorescence - Anti-beta III Tubulin Antibody [ARC0456] (A309019)
Immunofluorescence analysis of SH-SY5Y cells using Anti-beta III Tubulin Antibody [ARC0456] (A309019) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-beta III Tubulin Antibody [ARC0456] (A309019)
Immunofluorescence analysis of mouse brain using Anti-beta III Tubulin Antibody [ARC0456] (A309019) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-beta III Tubulin Antibody [ARC0456] (A309019)
Immunofluorescence analysis of rat brain using Anti-beta III Tubulin Antibody [ARC0456] (A309019) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Western Blot - Anti-beta III Tubulin Antibody [ARC0456] (A309019)
Immunoprecipitation analysis of 300µg extracts of mouse brain using 3µg of Anti-beta III Tubulin Antibody [ARC0456] (A309019). This Western blot was performed on the immunoprecipitate using Anti-beta III Tubulin Antibody [ARC0456] (A309019) at a dilution of 1:500.
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