Anti-beta Defensin 1 Antibody - BSA and Azide free
Description
Rabbit polyclonal antibody to beta Defensin 1.
Specificity
This antibody recognises human defensin beta 1 (BD-1), a secreted cationic antimicrobial peptide and member of the beta-defensin family, expressed at epithelial surfaces, which plays an important role in innate immunity.The activity of BD-1 is compromised in patients with Cystic Fibrosis due to salt-dependent inactivation.
Applications
WB, ELISA, IHC-P
Dilutions
ELISA: 0.5 - 2 µg/ml, WB: 0.1 - 0.2 µg/ml
Reactivity
Human
Immunogen
Recombinant human defensin beta-1.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Concentration
1 mg/ml after reconstitution.
Product Form
Lyophilized
Reconstitution
Reconstitute with 0.1 ml of distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Formulation
Supplied in Phosphate Buffered Saline.
Storage
Lyophilized: Store at -20°C. Reconstituted: Store undiluted at -20°C, avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Rabbit anti Human defensinß-1 polyclonal antibody recognizes human defensin beta 1 (BD-1), a secreted cationic antimicrobial peptide and member of the beta-defensin family, expressed at epithelial surfaces, which plays an important role in innate immunity.The activity of BD-1 is compromised in patients with Cystic Fibrosis due to salt-dependent inactivation (Goldman et al. 1997).
