Western Blot - Anti-beta Catenin Antibody (A80679)
Western blot analysis of extracts of various cell lines, using Anti-beta Catenin Antibody (A80679) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Western Blot - Anti-beta Catenin Antibody (A80679)
Western blot analysis of extracts of 293T cells, using Anti-beta Catenin Antibody (A80679) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.
Western Blot - Anti-beta Catenin Antibody (A80679)
Immunoprecipitation analysis of 300µg extracts of 293T cells using 3µg of Anti-beta Catenin Antibody (A80679). This Western blot was performed on the immunoprecipitate using Anti-beta Catenin Antibody (A80679) at a dilution of 1:1000.
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