Western Blot - Anti-beta Catenin Antibody (A12611)
Western blot analysis of extracts of Mouse lung, using Anti-beta Catenin Antibody (A12611) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-beta Catenin Antibody (A12611) at dilution of 20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human pancreas using Anti-beta Catenin Antibody (A12611) at dilution of 20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human small cell lung cancer using Anti-beta Catenin Antibody (A12611) at dilution of 20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Western Blot - Anti-beta Catenin Antibody (A12611)
Immunoprecipitation analysis of 600µg extracts of mouse brain using 3µg of Anti-beta Catenin Antibody (A12611). This Western blot was performed on the immunoprecipitate using Anti-beta Catenin Antibody (A12611) at a dilution of 1:1000.
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