Anti-beta Actin Antibody (A304904)

Western blot analysis of human HeLa and HEK293T cell lysates showing detection of ~41.7kDa beta Actin protein using Anti-beta Actin Antibody (A304904) at 1:1,000 for 1 hour at room temperature. Lane 1: MW Ladder. Lane 2: human HeLa (20 µg). Lane 3: human 293T (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at room temperature. The secondary antibody used was Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at room temperature. Color Development: TMB solution for 12 min at room temperature. Predicted/Observed Size: ~41.7kDa.

Immunocytochemistry/Immunofluorescence analysis of human cervical cancer cell line (HeLa), fixed in 4% formaldehyde for 15 min at room temperature, using Anti-beta Actin Antibody (A304904), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Rabbit ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:5,000 for 5 minutes room temperature. Localization: Cytoplasm, Cytoskeleton. Magnification: 40X.(A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Beta Actin Antibody (D) Composite.