Reconstitute with 100µl of sterile double-distilled water to bring antibody to 1mg/ml concentration. Gently shake to solubilize completely. Do not vortex!
Formulation
Lyophilized from 25mM Histadine, pH 6.2, with 8% Sucrose and 0.01% Tween80.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
SDS-PAGE analysis of Bemarituzumab Biosimilar - Anti-FGFR2 Antibody - Low endotoxin, Azide free (A323310) under reducing (R) conditions confirms that the purity of this antibody is greater than 95%.
SEC-HPLC analysis of Bemarituzumab Biosimilar - Anti-FGFR2 Antibody - Low endotoxin, Azide free (A323310) confirms that the purity of this antibody is 100%.
SNU-16 cells were stained with Bemarituzumab Biosimilar - Anti-FGFR2 Antibody - Low endotoxin, Azide free (A323310) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC50 = 0.5368 µg/ml.
Bemarituzumab Biosimilar - Anti-FGFR2 Antibody - Low endotoxin, Azide free (A323310) induced ADCC activity was evaluated using human FGFR2 HEK293 Reporter Cell. The max induction fold was approximately 25.
Bemarituzumab Biosimilar - Anti-FGFR2 Antibody - Low endotoxin, Azide free (A323310)-ADCC luciferase Assay on KATOIII cells. The maximum suppression factor is approximately 14.
Bemarituzumab Biosimilar - Anti-FGFR2 Antibody - Low endotoxin, Azide free (A323310) inhibited the tumor growth of SNU-16 on BALB/c nude mice. The result showed significant anti-tumor effects, with an tumor inhibition rate (TGI) of 29.7% at 2 mpk at D62.
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