Anti-Beclin 1 (phospho Ser93) Antibody (A308879)

Western blot analysis of extracts of HeLa cells, using Anti-Beclin 1 (phospho Ser93) Antibody (A308879) at 1:500 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.