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Anti-Beclin 1 (phospho Ser15) Antibody (A308398)

Western Blot - Anti-Beclin 1 (phospho Ser15) Antibody (A308398) - Antibodies.com
$435
Product Datasheet

Rabbit polyclonal antibody to Beclin 1 (phospho Ser15) for WB.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 5-8 business days.

  • Specifications

Name
Anti-Beclin 1 (phospho Ser15) Antibody
Description
Rabbit polyclonal antibody to Beclin 1 (phospho Ser15).
Applications
WB
Dilutions
WB: 1:100-1:500
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic phosphorylated peptide around S15 of human Beclin 1 (NP_003757.1).
Sequence
QVSFV
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
60 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Beclin-1, BECN1, Coiled-coil myosin-like BCL2-interacting protein, GT197, Protein GT197
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (1)

Western Blot - Anti-Beclin 1 (phospho Ser15) Antibody (A308398) - Antibodies.comEnlarge Image

Western Blot - Anti-Beclin 1 (phospho Ser15) Antibody (A308398)

Western blot analysis of extracts of various cell lines, using Anti-Beclin 1 (phospho Ser15) Antibody (A308398) at 1:500 dilution. NIH/3T3 and C6 cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
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