Recombinant Anti-BCMA Antibody [DM4] - BSA and Azide free (A318679)

ELISA plates were pre-coated with Recombinant Human BCMA Protein (Fc Chimera 6xHis Tag) (A318407), followed by pre-blocking with Human Anti-C11D5.3 Antibody (Grey bar) or Rabbit control IgG (Black bar), and then different Rabbit Antibodies were added to check the competitive inhibition of Human Anti-C11D5.3 Antibody. The clone DM3 exhibits the strongest inhibition (Red bar). This data indicated that DM3 bind to the same epitope as bb2121.

Immunoprecipitation analysis. Cellular overexpression lysates of HEK293F cells transfected with FLAG tagged human BCMA full length gene were pre-incubated with 6 different Rabbit Antibody clones and negative control IgG. The immunocomplexes were further pulled down by Protein A beads, fractionated, and blotted with Mouse Anti-FLAG Monoclonal Antibody.

Western Blot analysis of BCMA protein using Anti-BCMA Antibody [DM4] - BSA and Azide free (A318679) at 1:1000 dilution. Secondary Antibody: Goat Anti-Rabbit IgG (H+L) (HRP) at 1:5000 dilution. 1. K562 cell lysate (negative control). 2. K562 cell lysate with overexpressed human BCMA proteins. 3. MM.1S cell lysate (native BCMA protein).

Flow cytometry analysis of antigen binding of Anti-BCMA Antibody [DM4] - BSA and Azide free (A318679). (A) Anti-BCMA Antibody [DM4] - BSA and Azide free (A318679) does not bind to Jurkat cells that do not express BCMA. (B) A clear peak shift of Anti-BCMA Antibody [DM4] - BSA and Azide free (A318679) was seen compared to the control when incubated with BCMA-expressing MM.1S cells, indicating strong binding of Anti-BCMA Antibody [DM4] - BSA and Azide free (A318679) to BCMA. Antibodies were incubated at 2 µg/ml. .