Anti-Bcl-2 Antibody [SPM117] (A249865)

Anti-Bcl-2 Antibody [SPM117]

Mouse monoclonal [SPM117] antibody to Bcl-2.

This antibody recognizes a protein of 25-26kDa, identified as the bcl-2 lpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2 lpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 lpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.

Flow Cytometry, IF, WB, IHC-P

Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml, WB: 1-2 µg/ml, IHC-P: 1-2 µg/ml

Human

This antibody does not cross react with Mouse or Rat.

Synthetic peptide corresponding to amino acids 41-54 of human Bcl-2 protein.

GAAPAPGIFSSQPG-Cys

Mouse

Monoclonal

SPM117

IgG1

Protein A/G chromatography.

200 µg/ml

25-26 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-Bcl-2 Antibody [SPM117] - BSA and Azide free (A253045).

Apoptosis regulator Bcl-2, BCL2