Mouse monoclonal [Bcl-2/100] antibody to Bcl-2 (FITC).
Specificity
This antibody recognizes Bcl2, a 26 kDa protooncogen with anti-apoptotic effect, expressed in outer mitochondrial membrane, endoplasmic reticulum and nuclear envelope.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 4 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests; Intracellular staining.
Reactivity
Human
Cross Reactivity
This antibody does not cross react with Mouse.
Immunogen
Synthetic peptide corresponding to the amino acids 41-54 of human Bcl2.
Host
Mouse
Clonality
Monoclonal
Clone ID
Bcl-2/100
Isotype
IgG1
Conjugate
FITC
Excitation: 490nm, Emission: 525nm
Purification
This antibody is conjugated with Fluorescein Isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Predicted MW
26 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!