Anti-Bcl-2 Antibody (A12540)

Name: Anti-Bcl-2 Antibody
Brand: Antibodies.com
SKU: A12540
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to Bcl-2 for WB, IHC, ICC/IF and IP.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-Bcl-2 Antibody

Description

Rabbit polyclonal antibody to Bcl-2.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Bcl-2 (NP_000624.2).

Sequence

MAHAGRTGYDNREIVMKYIHYKLSQRGYEWDAGDVGAAPPGAAPAPGIFSSQPGHTPHPAASRDPVARTSPLQTPAAPGAAAGPALSPVPPVVHLTLRQA

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

26 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Apoptosis regulator Bcl-2, BCL2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-Bcl-2 Antibody (A12540)

Western blot analysis of 293T, using Anti-Bcl-2 Antibody (A12540) at 1:900 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Immunohistochemistry - Anti-Bcl-2 Antibody (A12540)

Immunohistochemistry analysis of paraffin-embedded human appendix tissue using Anti-Bcl-2 Antibody (A12540) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunofluorescence - Anti-Bcl-2 Antibody (A12540)

Immunofluorescence analysis of NIH/3T3 cells using Anti-Bcl-2 Antibody (A12540) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Western Blot - Anti-Bcl-2 Antibody (A12540)

Immunoprecipitation analysis of 200µg extracts of THP-1 cells using 3µg of Anti-Bcl-2 Antibody (A12540). This Western blot was performed on the immunoprecipitate using Anti-Bcl-2 Antibody (A12540) at a dilution of 1:1000.

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