Anti-Bcl-2 Antibody [8C8] - BSA and Azide free (A253046)

Anti-Bcl-2 Antibody [8C8] - BSA and Azide free

Mouse monoclonal [8C8] antibody to Bcl-2.

This antibody recognizes a protein of 25-26kDa, identified as the bcl-2 lpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2 lpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 lpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.

Flow Cytometry, IF, WB, IHC-P

Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml, WB: 1-2 µg/ml, IHC-P: 1-2 µg/ml

Human, Monkey, Porcine

This antibody does not cross react with Mouse and Rat.

Synthetic peptide corresponding to amino acids 41-54 of human Bcl-2 protein.

GAAPAPGIFSSQPG-Cys

Mouse

Monoclonal

8C8

IgG1

Protein A/G chromatography.

1 mg/ml

25-26 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline; without Sodium Azide and carrier free.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

This monoclonal antibody is also available in a different formulation with BSA and Sodium Azide - Anti-Bcl-2 Antibody [8C8] (A249866).

Apoptosis regulator Bcl-2, BCL2