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Anti-Bcl-2 Antibody [100] (A278770)

$350

Mouse monoclonal [100] antibody to Bcl-2 for WB, IHC-P, Flow Cytometry, IHC-Fr and IP.

Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 5-7 business days.
Name
Anti-Bcl-2 Antibody [100]
Description
Mouse monoclonal [100] antibody to Bcl-2.
Specificity
This antibody recognises the human Apoptosis regulator Bcl-2 oncoprotein, a 239 amino acid ~25kDa integral single pass membrane protein containing 4 BH motifs which lies within the cell rather than on the cell surface. The protein is localised in the outer mitochondrial membrane and plays a role in the inhibition of apoptosis. Mouse anti Human Bcl-2, clone 100 reacts with small B lymphocytes in the mantle zone and many cells within T cell areas. Very few cells in germinal centres are stained. In the thymus many cells in the medulla are stained but the cortex shows weak or negative staining. In non-haematopoietic tissues few cells are stained. Mouse anti Human Bcl-2 antibody, clone 100 reacts with neoplastic cells of follicular lymphoma and anaplastic large cell lymphoma (Reed 1997). Mouse anti human Bcl-2, clone 100 has been successfully used to demonstrate Bcl-2 in formalin fixed, paraffin embedded tissues of radioresistant squamous cell carcinoma by immunohistochemistry.
Applications
WB, IHC-P, Flow Cytometry, IHC-Fr, IP
Dilutions
FC 1: 1:50 - 1:100, IHC-Fr: 1:20 - 1:40, IHC-P 2: 1:20 - 1:40, Use 10µl of the suggested working dilution to label 1x106 cells in 100µl.
Reactivity
Human
Immunogen
Synthetic peptide corresponding to amino acids 41-54 of the Bcl-2 protein.
Sequence
GAAPAPGIFSSQPG
Host
Mouse
Clonality
Monoclonal
Clone ID
100
Isotype
IgG1
Conjugate

Unconjugated

Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Mouse anti Human Bcl-2 antibody, clone 100 recognizes the human Apoptosis regulator Bcl-2 oncoprotein, a 239 amino acid ~25kDa integral single pass membrane protein containing 4 BH motifs which lies within the cell rather than on the cell surface. The protein is localised in the outer mitochondrial membrane and plays a role in the inhibition of apoptosis. Mouse anti Human Bcl-2, clone 100 reacts with small B lymphocytes in the mantle zone and many cells within T cell areas. Very few cells in germinal centres are stained. In the thymus many cells in the medulla are stained but the cortex shows weak or negative staining. In non-haematopoietic tissues few cells are stained (Krajewski et al. 1995). Mouse anti Human Bcl-2 antibody, clone 100 reacts with neoplastic cells of follicular lymphoma and anaplastic large cell lymphoma (Reed 1997).Mouse anti human Bcl-2, clone 100 has been successfully used to demonstrate Bcl-2 in formalin fixed, paraffin embedded tissues of radioresistant squamous cell carcinoma by immunohistochemistry (Condon et al. 2002).
Synonyms
Apoptosis regulator Bcl-2, BCL2
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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