This antibody recognizes a protein of 21kDa, identified as the Bax protein. This MAb is highly specific to Bax and shows no cross-reaction with Bcl-2 or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2.
Flow cytometric analysis of human Jurkat cells using Anti-Bax Antibody [SPM336] followed by Goat Anti-Mouse IgG (CF® 488) (Blue). Isotype Control (Red).
Immunofluorescent analysis of Jurkat cells stained with Anti-Bax Antibody [SPM336] followed by Goat Anti-Mouse IgG (CF® 488) (Green). The nuclear counterstain is NucSpot.
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