Recombinant rabbit monoclonal [BAX/8960R] antibody to Bax.
Specificity
Recognizes a protein of 21kDa, identified as the Bax protein. This MAb is highly specific to Bax and shows no cross-reaction with Bcl-2 or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2.
Applications
IHC-P
Positive Control
Jurkat, K562, HL-60, or HeLa cells. Reed-Sternberg cells in Hodgkin s lymphoma.
Dilutions
IHC: 1-2 µg/ml
Reactivity
Human
Immunogen
Recombinant full-length human BAX protein.
Host
Rabbit
Clonality
Monoclonal
Clone ID
BAX/8960R
Isotype
IgG
Light Chains
kappa
Conjugate
Unconjugated
Purification
Protein A/G chromatography.
Concentration
200 µg/ml
Molecular Weight
21 kDa
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Apoptosis regulator BAX, Bcl-2-like protein 4, Bcl2-L-4, BCL2L4
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