Anti-Bax Antibody (A81180)

Name: Anti-Bax Antibody
Brand: Antibodies.com
SKU: A81180
Price: 470.0 USD
Availability: InStock

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$470

Rabbit polyclonal antibody to Bax for WB, ICC/IF and IP.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Tags:

Knockout Validated Antibody

Specifications

Name

Anti-Bax Antibody

Description

Rabbit polyclonal antibody to Bax.

Applications

WB, ICC/IF, IP

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:100, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human Bax (NP_620116.1).

Sequence

MDGSGEQPRGGGPTSSEQIMKTGALLLQGFIQDRAGRMGGEAPELALDPVPQDASTKKLSECLKRIGDELDSNMELQRMIAAVDTDSPREVFFRVAADMF

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

23 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Apoptosis regulator BAX, Bcl-2-like protein 4, Bcl2-L-4, BCL2L4

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-Bax Antibody (A81180)

Western blot analysis of extracts of various cell lines, using Anti-Bax Antibody (A81180) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Western Blot - Anti-Bax Antibody (A81180)

Western blot analysis of extracts from normal (control) and Bax knockout (KO) 293T cells, using Anti-Bax Antibody (A81180) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.

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Western Blot - Anti-Bax Antibody (A81180)

Immunoprecipitation analysis of 200µg extracts of HeLa cells using 3µg of Anti-Bax Antibody (A81180). This Western blot was performed on the immunoprecipitate using Anti-Bax Antibody (A81180) at a dilution of 1:1000.

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