Western blot analysis of rat kidney showing detection of ~18.3 kDa BAD protein using Anti-Bad Antibody (A305211) at 1:1,000 for 2 hours at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: rat Kidney. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Rabbit IgG: HRP at 1:3000 for 1 hour at room temperature. Color Development: ECL solution for 5 minutes at room temperature. Predicted/Observed Size: ~18.3 kDa. Other Band(s): 28 kDa (PTM).