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Anti-B-Raf (phospho Thr753) Antibody (A93878)

Western Blot - Anti-B-Raf (phospho Thr753) Antibody (A8305) - Antibodies.com
$190
Product Datasheet

Rabbit polyclonal antibody to B-Raf (phospho Thr753) for WB and ELISA.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 6-8 business days.

  • Specifications

Name
Anti-B-Raf (phospho Thr753) Antibody
Description
Rabbit polyclonal antibody to B-Raf (phospho Thr753).
Specificity
This antibody detects endogenous levels of B-Raf only when phosphorylated at Thr753.
Applications
WB, ELISA
Dilutions
WB: 1:500-1:1000, IF: 1:100-1:500, ELISA: 1:5000
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide derived from human B-Raf around the phosphorylation site of Thr753 (amino acids 717-766).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Molecular Weight
84kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
BRAF, BRAF1, p94, Proto-oncogene B-Raf, RAFB1, Serine/threonine-protein kinase B-raf, v-Raf murine sarcoma viral oncogene homolog B1
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (1)

Western Blot - Anti-B-Raf (phospho Thr753) Antibody (A8305) - Antibodies.comEnlarge Image

Western Blot - Anti-B-Raf (phospho Thr753) Antibody (A93878)

Western blot analysis of lysates from K562 cells treated with EGF 200ng/ml 30' using Anti-B-Raf (phospho Thr753) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.
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