Anti-Aurora B Kinase Antibody [3F11] (A85381)

High magnification confocal immunofluorescence of HeLa cells stained with Anti-Aurora B Kinase Antibody, at a dilution of 1:1,000, in green, and Anti-Vimentin Antibody (A85421 | 1:1,000, in red. Blue is the DNA stain DAPI revealing the nuclei. The Anti-Aurora B Kinase Antibody reveals aurora B localized in midbodies, midzones of dividing cells and also in the nuclei or some cells.

HeLa cell cultures were stained with Anti-Aurora B Kinase Antibody (green). Anti-Aurora B Kinase Antibody stains midzones in anaphase and midbodies between the two daughter cells during telophase. It is therefore a useful marker of dividing cells. Cells were counterstained with Anti-Vimentin Antibody (A85421 | red). Blue is a DNA stain.

Western blot analysis of different cell lysates and recombinant protein solutions using Anti-Aurora B Kinase Antibody [3F11] (A85381), in green. Left: Cells were treated with 100 ng/ml of nocodazol, a microtubule depolymerizing agent which induces cells to halt at G2/M phase. The lanes contain samples of: [Lane 1] Protein standards, in red, [Lane 2] HeLa cells, [Lane 3] canine A72 cells, [Lane 4] equine NBL6 cells, and [Lane 5] mouse KR158 cells. Right: Western blot of purified full-length recombinant human Aurora A, B and C, probed with Anti-Aurora B Kinase Antibody [3F11] (A85381). The antibody binds specifically only to Aurora B and not to the closely related Aurora A and C, shown by the single band in lane B.

Left: Western blot analysis of Anti-Aurora B Kinase Antibody on lysateas from HeLa cells treated with 100ng/ml nocodazole for 18 hours. Nocodazole is a microtubule polymerization inhibitor which induces cells to halt at the G2/M phase and also induces Aurora B expression. The Anti-Aurora B Kinase Antibody binds strongly to the aurora B band at 38 kDa. Right: Blots of recombinant human Aurora A, B and C were probed with Anti-Aurora B Kinase Antibody. This antibody binds specifically only to aurora B and not to the closely related aurora A and C. This is also consistent with the immunocytochemical staining of midzones and midbodies on HeLa cells.