Anti-Aurora B Antibody [ARC50905] (A306974)

Name: Anti-Aurora B Antibody [ARC50905]
Brand: Antibodies.com
SKU: A306974
Price: 485.0 USD
Availability: InStock

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$485

Rabbit monoclonal [ARC50905] antibody to Aurora B for WB and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-Aurora B Antibody [ARC50905]

Description

Rabbit monoclonal [ARC50905] antibody to Aurora B.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Aurora B (NP_004208.2).

Sequence

MAQKENSYPWPYGRQTAPSGLSTLPQRVLRKEPVTPSALVLMSRSNVQPTAAPGQKVMENSSGTPDILTRHFTIDDFEIGRPLGKGKFGNVYLAREKKSH

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC50905

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

39 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

AIK2, AIM-1, AIM1, AIRK2, ARK-2, ARK2, AURKB, Aurora 1, Aurora kinase B, Aurora- and IPL1-like midbody-associated protein 1, Aurora-related kinase 2, Aurora/IPL1-related kinase 2, Serine/threonine-protein kinase 12, STK-1, STK1, STK12, STK5

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Western Blot - Anti-Aurora B Antibody [ARC50905] (A306974)

Western blot analysis of extracts of various cell lines, using Anti-Aurora B Antibody [ARC50905] (A306974) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.

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Immunofluorescence - Anti-Aurora B Antibody [ARC50905] (A306974)

Confocal imaging of HeLa cells using Anti-Aurora B Antibody [ARC50905] (A306974), at a dilution of 1:800, (red). The cells were counterstained with Anti-Vimentin Antibody, at a dilution of 1:100, (green). DAPI was used for nuclear staining (Blue). Objective: 60x.

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