Anti-Aurora A (phospho Thr288) Antibody (A11002)

Western blot analysis of extracts of HeLa and HT-29 cells, using Anti-Aurora A (phospho Thr288) Antibody (A11002) at 1:1,000 dilution. HeLa cells were treated by Hydroxyurea (4mM) for 20 hours. HeLa cells were treated by Nocodazole (50ng/ml) for 20 hours. HT-29 cells were treated by Hydroxyurea (4 mM) for 20 hours or treated by Nocodazole (100ng/ml) for 16 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 90s.