Anti-Aurora A (phospho Thr288) Antibody (A11002)

Name: Anti-Aurora A (phospho Thr288) Antibody
Brand: Antibodies.com
SKU: A11002
Price: 435.0 USD
Availability: InStock

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$435

Rabbit polyclonal antibody to Aurora A (phospho Thr288) for WB.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-Aurora A (phospho Thr288) Antibody

Description

Rabbit polyclonal antibody to Aurora A (phospho Thr288).

Applications

Dilutions

WB: 1:500-1:2,000

Reactivity

Human

Immunogen

A synthetic phosphorylated peptide around T288 of human AURKA (NP_003591.2).

Sequence

RTTLC

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

48 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

AIK, AIRK1, ARK-1, ARK1, AURA, AURKA, Aurora 2, Aurora kinase A, Aurora-related kinase 1, Aurora/IPL1-related kinase 1, AYK1, Breast tumor-amplified kinase, BTAK, IAK1, Ipl1- and aurora-related kinase 1, Serine/threonine-protein kinase 15, STK15, STK6

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (1)

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Western Blot - Anti-Aurora A (phospho Thr288) Antibody (A11002)

Western blot analysis of extracts of HeLa and HT-29 cells, using Anti-Aurora A (phospho Thr288) Antibody (A11002) at 1:1,000 dilution. HeLa cells were treated by Hydroxyurea (4mM) for 20 hours. HeLa cells were treated by Nocodazole (50ng/ml) for 20 hours. HT-29 cells were treated by Hydroxyurea (4 mM) for 20 hours or treated by Nocodazole (100ng/ml) for 16 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 90s.

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