Anti-Aurora A + B + C Kinase Antibody [4A7] (A85401)

Name: Anti-Aurora A + B + C Kinase Antibody [4A7]
Brand: Antibodies.com
SKU: A85401
Price: 185.0 USD
Availability: InStock

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$185

Mouse monoclonal (4A7) antibody to Aurora A + B + C Kinase for WB, ICC/IF and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 4-6 business days.

Specifications

Name

Anti-Aurora A + B + C Kinase Antibody [4A7]

Description

Mouse monoclonal (4A7) antibody to Aurora A + B + C Kinase.

Specificity

This antibody was raised against human Aurora A protein and was shown to also bind Aurora B and Aurora C; as a result the epitope is likely within the highly conserved serine/threonine kinase domain. This antibody can be used to identify dividing or soon to be dividing cells and is also an excellent marker of midbodies both during and after cell division.

Applications

WB, ICC/IF, IHC

Dilutions

WB: 1:1,000, ICC/IF: 1:1,000-1:2,000, IHC: 1:1,000-1:2,000

Reactivity

Human, Horse, Bovine, Porcine, Rat, Mouse

Immunogen

Recombinant full-length human Aurora A protein, expressed in and purified from E. coli.

Host

Mouse

Clonality

Monoclonal

Clone ID

4A7

Isotype

IgG1

Conjugate

Unconjugated

Purification

Immunogen affinity purification.

Concentration

1 mg/ml

Molecular Weight

46kDa, 38kDa, 35kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 50% Glycerol and 5mM Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

AIK, AIRK1, ARK-1, ARK1, AURA, AURKA, Aurora 2, Aurora kinase A, Aurora-related kinase 1, Aurora/IPL1-related kinase 1, AYK1, Breast tumor-amplified kinase, BTAK, IAK1, Ipl1- and aurora-related kinase 1, Serine/threonine-protein kinase 15, STK15, STK6

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Immunofluorescence - Anti-Aurora A + B + C Kinase Antibody [4A7] (A85401)

HeLa cell cultures were stained with Anti-Aurora A/B/C Kinase Antibody (green). In this image, strong staining in spindle poles was seen in cells at anaphase. This antibody also stains the midbodies between the two daughter cells. Cells were counterstained with Anti-Vimentin Antibody (A85421 | red). Blue is a DNA stain.

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Western Blot - Anti-Aurora A + B + C Kinase Antibody [4A7] (A85401)

Left: Western blot analysis of Anti-Aurora A/B/C Kinase Antibody in HeLa cells. Blot of HeLa cells treated with 100ng/ml nocodazole for 18 hours was probed with Anti-Aurora A/B/C Kinase Antibody. Nocodazole is a microtubule depolymerizating agent which induces cells to halt at the G2/M phase and also induces Aurora kinase expression. The Anti-Aurora A/B/C Kinase Antibody binds strongly to bands at about 46 kDa and 38kDa, corresponding to Aurora A and Aurora B. Also, it recognizes a weak band at 35 kDa which is Aurora C. Right: Blot of recombinant full length human Aurora A, B and C was probed with Anti-Aurora A/B/C Kinase Antibody. This antibody reacts strongly with all three Aurora kinases proteins.

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