Anti-ATR (phospho Ser428) Antibody (A91791)

Name: Anti-ATR (phospho Ser428) Antibody
Brand: Antibodies.com
SKU: A91791
Price: 485.0 USD
Availability: InStock

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$485

Rabbit polyclonal antibody to ATR (phospho Ser428) for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-ATR (phospho Ser428) Antibody

Description

Rabbit polyclonal antibody to ATR (phospho Ser428).

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:100-1:500, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around S428 of human ATR (NP_001175.2).

Sequence

GISPK

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

300 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Ataxia telangiectasia and Rad3-related protein, FRAP-related protein 1, FRP1, Serine/threonine-protein kinase ATR

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (6)

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Western Blot - Anti-ATR (phospho Ser428) Antibody (A91791)

Western blot analysis of 293T, using Anti-ATR (phospho Ser428) Antibody (A91791) at 1:400 dilution. 293T cells were treated by UV at room temperature for 15-30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Western Blot - Anti-ATR (phospho Ser428) Antibody (A91791)

Western blot analysis of NIH/3T3, using Anti-ATR (phospho Ser428) Antibody (A91791) at 1:400 dilution. NIH/3T3 cells were treated by Nocodazole (50 ng/ml) at 37°C for 20 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Immunohistochemistry - Anti-ATR (phospho Ser428) Antibody (A91791)

Immunohistochemistry analysis of paraffin-embedded human testis using Anti-ATR (phospho Ser428) Antibody (A91791) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-ATR (phospho Ser428) Antibody (A91791)

Immunohistochemistry analysis of paraffin-embedded mouse testis using Anti-ATR (phospho Ser428) Antibody (A91791) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.