Western Blot - Anti-ATP5A Antibody [ARC0549] (A309098)
Western blot analysis of extracts of various cell lines, using Anti-ATP5A Antibody [ARC0549] (A309098) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-ATP5A Antibody [ARC0549] (A309098) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-ATP5A Antibody [ARC0549] (A309098) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse spleen using Anti-ATP5A Antibody [ARC0549] (A309098) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Confocal imaging of NIH/3T3 cells using Anti-ATP5A Antibody [ARC0549] (A309098), at a dilution of 1:100, (green). The cells were counterstained with Anti-alpha Tubulin Antibody, at a dilution of 1:100, (red). DAPI was used for nuclear staining (Blue). Objective: 60x.
Western Blot - Anti-ATP5A Antibody [ARC0549] (A309098)
Immunoprecipitation analysis of 600µg extracts of mouse heart cells using 3µg of Anti-ATP5A Antibody [ARC0549] (A309098). This Western blot was performed on the immunoprecipitate using Anti-ATP5A Antibody [ARC0549] (A309098) at a dilution of 1:1000.