Anti-ATP1B1 Antibody [ARC1232] (A309240)

Name: Anti-ATP1B1 Antibody [ARC1232]
Brand: Antibodies.com
SKU: A309240
Price: 485.0 USD
Availability: InStock

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$485

Rabbit monoclonal [ARC1232] antibody to ATP1B1 for WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-ATP1B1 Antibody [ARC1232]

Description

Rabbit monoclonal [ARC1232] antibody to ATP1B1.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 204-303 of human ATP1B1 (P05026).

Sequence

YNPNVLPVQCTGKRDEDKDKVGNVEYFGLGNSPGFPLQYYPYYGKLLQPKYLQPLLAVQFTNLTMDTEIRIECKAYGENIGYSEKDRFQGRFDVKIEVKS

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1232

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

50 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

ATP1B, Sodium/potassium-dependent ATPase subunit beta-1, Sodium/potassium-transporting ATPase subunit beta-1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-ATP1B1 Antibody [ARC1232] (A309240)

Western blot analysis of extracts of various cell lines, using Anti-ATP1B1 Antibody [ARC1232] (A309240) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Western Blot - Anti-ATP1B1 Antibody [ARC1232] (A309240)

Western blot analysis of extracts of U2OS cells, using Anti-ATP1B1 Antibody [ARC1232] (A309240) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 30s.

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Immunohistochemistry - Anti-ATP1B1 Antibody [ARC1232] (A309240)

Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-ATP1B1 Antibody [ARC1232] (A309240) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-ATP1B1 Antibody [ARC1232] (A309240)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-ATP1B1 Antibody [ARC1232] (A309240) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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