This antibody recognises human Autophagy-related protein 12 (ATG12).ATG12, a member of the autophagy protein family, forms a conjugate with ATG5; this conjugate has a ubiquitin-protein ligase (E3)-like activity for protein lipidation in autophagy. This conjugate also associates with innate immune response proteins such as RIG-I and VISA, inhibiting type I interferon production and permitting viral replication in host cells. ATG12 has also been shown to interact with ATG10 in human embryonic kidney cells in the presence of ATG7.There are at least two isoforms of ATG12.
Applications
WB, IHC-P
Dilutions
IHC-P: 2.5 µg/ml, WB: 0.5 - 1 µg/ml
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide corresponding to 16 amino acids near the N-terminus of human ATG12.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Concentration
1 mg/ml
Molecular Weight
Approximately 40 kDa in human brain tissue lysate.
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.02% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Rabbit anti ATG12 (N-Terminal) antibody recognizes human Autophagy-related protein 12 (ATG12).ATG12, a member of the autophagy protein family, forms a conjugate with ATG5; this conjugate has a ubiquitin-protein ligase (E3)-like activity for protein lipidation in autophagy. This conjugate also associates with innate immune response proteins such as RIG-I and VISA, inhibiting type I interferon production and permitting viral replication in host cells. ATG12 has also been shown to interact with ATG10 in human embryonic kidney cells in the presence of ATG7.There are at least two isoforms of ATG12.
Synonyms
APG12, APG12-like, APG12L, Autophagy-related protein 12, Ubiquitin-like protein ATG12