ATF6 expression in various lysates analyzed by western blot. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-ATF6 Antibody (A91443) at a dilution of 1:1000 and detected with chemiluminescence.
ATF6 expression in rat kidney tissue analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved with 10 mM citrate buffer, pH 6.0, under high pressure. Staining was performed with Anti-ATF6 Antibody (A91443) at a dilution of 1:200.
ATF6 expression in Hela cells analyzed by immunofluorescence. Staining was performed with Anti-ATF6 Antibody (A91443) at a dilution of 1:100 followed by Cy3 Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:500. Nuclei were stained with DAPI (blue).
ATF6 expression in NIH/3T3 cells analyzed by immunofluorescence. Staining was performed with Anti-ATF6 Antibody (A91443) at a dilution of 1:100 followed by Cy3 Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:500. Nuclei were stained with DAPI (blue).
ATF6 expression in PC-12 cells analyzed by immunofluorescence. Staining was performed with Anti-ATF6 Antibody (A91443) at a dilution of 1:100 followed by Cy3 Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:500. Nuclei were stained with DAPI (blue).
ATF6 expression in PC-12 cells analyzed by immunofluorescence. Staining was performed with Anti-ATF6 Antibody (A91443) at a dilution of 1:200 followed by Cy3 Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:500. Nuclei were stained with DAPI (blue).