Anti-ATF2 (phospho Thr52) Antibody (A283326)

Anti-ATF2 (phospho Thr52) Antibody

Rabbit polyclonal antibody to ATF2 (phospho Thr52).

This antibody recognises activating transcription factor 2 (ATF2), also known as cyclic AMP-dependent transcription factor ATF-2 and cyclic AMP-responsive element-binding protein 2 (CREB-2), when phosphorylated at threonine 52.The ATF2 gene encodes a transcription factor that is a member of the leucine zipper family of DNA-binding proteins. ATF2 binds to the cAMP-responsive element (CRE), an octameric palindrome. The protein forms a homodimer or heterodimer with c-Jun and stimulates CRE-dependent transcription. There is abnormal activation of ATF-2 in growth and progression of mammalian skin tumors.ATF2 may mediate oncogenesis caused by mutant Ras protein and regulate maintenance of the aggressive cancer phenotype of some types of epithelial cells. ATF2 has also been shown to be phosphorylated by ATM at Serine 490 and 498 in humans. The serine phosphorylated forms play a role in mediating DNA repair and in response to DNA damage induced by ionizing radiation (IR).ATF2 exhibits histone acetyltransferase (HAT) activity and has been reported to specifically acetylate histones H2B and H4 in vitro. In concert with CUL3 and RBX1, ATF2 promotes the degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM. This protein can also elicit oncogenic or tumor suppressor activities depending on the tissue or cell type. In response to stress stimuli, ATF2 activates a variety of gene targets that are involved in oncogenesis, and has been correlated with maintenance of a cancer cell phenotype. Inhibiting ATF2 impedes melanoma development and elicits tumor suppressor function. To act as a tumor suppressor, ATF2 must localize at the mitochondria and phosphorylation at Thr52 by PKCe regulates this translocation.

WB: 1:250

Human, Bovine, Chicken, Mouse, Primate, Rat, Xenopus

Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr52 region of human ATF2.

Rabbit

Polyclonal

IgG

Approximately 72 kDa in UACC903 melanoma cell line lysate

Liquid

Supplied in HEPES Buffered Saline with 1% BSA, 50% Glycerol and 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Rabbit anti ATF2 (pThr52) antibody recognizes activating transcription factor 2 (ATF2), also known as cyclic AMP-dependent transcription factor ATF-2 and cyclic AMP-responsive element-binding protein 2 (CREB-2), when phosphorylated at threonine 52.The ATF2 gene encodes a transcription factor that is a member of the leucine zipper family of DNA-binding proteins. ATF2 binds to the cAMP-responsive element (CRE), an octameric palindrome. The protein forms a homodimer or heterodimer with c-Jun and stimulates CRE-dependent transcription. There is abnormal activation of ATF-2 in growth and progression of mammalian skin tumors.ATF2 may mediate oncogenesis caused by mutant Ras protein and regulate maintenance of the aggressive cancer phenotype of some types of epithelial cells. ATF2 has also been shown to be phosphorylated by ATM at Serine 490 and 498 in humans. The serine phosphorylated forms play a role in mediating DNA repair and in response to DNA damage induced by ionizing radiation (IR).ATF2 exhibits histone acetyltransferase (HAT) activity and has been reported to specifically acetylate histones H2B and H4 in vitro. In concert with CUL3 and RBX1, ATF2 promotes the degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM. This protein can also elicit oncogenic or tumor suppressor activities depending on the tissue or cell type. In response to stress stimuli, ATF2 activates a variety of gene targets that are involved in oncogenesis, and has been correlated with maintenance of a cancer cell phenotype. Inhibiting ATF2 impedes melanoma development and elicits tumor suppressor function. To act as a tumor suppressor, ATF2 must localize at the mitochondria and phosphorylation at Thr52 by PKCe regulates this translocation.

Activating transcription factor 2, cAMP response element-binding protein CRE-BP1, cAMP-dependent transcription factor ATF-2, cAMP-responsive element-binding protein 2, CREB-2, CREB2, CREBP1, Cyclic AMP-dependent transcription factor ATF-2, Cyclic AMP-responsive element-binding protein 2, HB16