Recombinant humanized monoclonal antibody to PD-L1.
Applications
ELISA, FACS, Functional Assay, In Vivo
Reactivity
Human, Cynomolgus Macaque
Host
Humanized
Clonality
Monoclonal
Isotype
IgG1
Light Chains
kappa
Conjugate
Unconjugated
Purification
Protein A affinity chromatography.
Concentration
Reconstitution dependent.
Molecular Weight
This antibody has a predicted MW of 145 kDa.
Purity
> 95% (by SDS-PAGE and SEC-HPLC).
Product Form
Lyophilized
Reconstitution
Reconstitute with 100µl of sterile double-distilled water to bring antibody to 1mg/ml concentration. Gently shake to solubilize completely. Do not vortex!
Formulation
Lyophilized from 25mM Histadine, pH 6.2, with 8% Sucrose and 0.01% Tween80.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
SDS-PAGE analysis of Atezolizumab Biosimilar - Anti-PD-L1 Antibody - Low endotoxin, Azide free (A323290) under reducing (R) conditions confirms that the purity of this antibody is greater than 95%.
SEC-HPLC analysis of Atezolizumab Biosimilar - Anti-PD-L1 Antibody - Low endotoxin, Azide free (A323290) confirms that the purity of this antibody is 100%.
Human PD-L1 CHO-K cells were stained with Atezolizumab Biosimilar - Anti-PD-L1 Antibody - Low endotoxin, Azide free (A323290) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC50 = 1.533 nM.
The endocytosis ratio of Atezolizumab Biosimilar - Anti-PD-L1 Antibody - Low endotoxin, Azide free (A323290) by HCC827 increased with the increase of antibody concentration, and the Internalization Rate (%) reached 60% at antibody concentration of 0.5 nM.
Atezolizumab Biosimilar - Anti-PD-L1 Antibody - Low endotoxin, Azide free (A323290) inhibited the tumor growth of MC38 on C57BL/6N mice. The result showed significant anti-tumor effects, with an tumor inhibition rate (TGI) of 85.2% at 2 mpk at D35.
Atezolizumab Biosimilar - Anti-PD-L1 Antibody - Low endotoxin, Azide free (A323290) has an affinity constant of 7.62E-11M as determined in BLI assay, using human B7-H1 / PD-L1 / CD274, His Tag.
Publishing research using Atezolizumab Biosimilar - Anti-PD-L1 Antibody - Low endotoxin, Azide free (A323290)? Please let us know so that we can list the citation on this page.
Alternative products to Atezolizumab Biosimilar - Anti-PD-L1 Antibody - Low endotoxin, Azide free (A323290)
![Immunohistochemistry - Anti-PD-L1 Antibody [PDL1/2746] (A248773) - Antibodies.com](https://cdn.antibodies.com/image/catalog/248/A248773_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-PD-L1 Antibody [PDL1/2746] - BSA and Azide free (A251953) - Antibodies.com](https://cdn.antibodies.com/image/catalog/251/A251953_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-PD-L1 Antibody [PDL1/4451R] (A248775) - Antibodies.com](https://cdn.antibodies.com/image/catalog/248/A248775_1.jpg?profile=product_alternative)
![Western Blot - Anti-PD-L1 Antibody [ARC5110-01] (A93289) - Antibodies.com](https://cdn.antibodies.com/image/catalog/93/A93289_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-PD-L1 Antibody [PDL1/4451R] - BSA and Azide free (A251955) - Antibodies.com](https://cdn.antibodies.com/image/catalog/251/A251955_1.jpg?profile=product_alternative)
