Anti-ASH2L Antibody [ARC0326] (A305306)

Name: Anti-ASH2L Antibody [ARC0326]
Brand: Antibodies.com
SKU: A305306
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC0326] antibody to ASH2L for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Tags:

Nuclear Marker

Specifications

Name

Anti-ASH2L Antibody [ARC0326]

Description

Rabbit monoclonal [ARC0326] antibody to ASH2L.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 529-628 of human ASH2L (Q9UBL3).

Sequence

AEKSLKQTPHSEIIFYKNGVNQGVAYKDIFEGVYFPAISLYKSCTVSINFGPCFKYPPKDLTYRPMSDMGWGAVVEHTLADVLYHVETEVDGRRSPPWEP

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0326

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

65 kDa / 80 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

ASH2-like protein, ASH2L1, Set1/Ash2 histone methyltransferase complex subunit ASH2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (7)

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Western Blot - Anti-ASH2L Antibody [ARC0326] (A305306)

Western blot analysis of extracts of various cell lines, using Anti-ASH2L Antibody [ARC0326] (A305306) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.

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Immunohistochemistry - Anti-ASH2L Antibody [ARC0326] (A305306)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-ASH2L Antibody [ARC0326] (A305306) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-ASH2L Antibody [ARC0326] (A305306)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-ASH2L Antibody [ARC0326] (A305306) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-ASH2L Antibody [ARC0326] (A305306)

Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-ASH2L Antibody [ARC0326] (A305306) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.