Synthetic peptide within amino acids 50-100 of human ASH2L (NP_004665. 1).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Antigen affinity purification.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Tris-Citrate/Phosphate Buffer, pH 7-8, with 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
General Notes
This antibody was affinity purified using the immunising peptide immobilized on solid support. Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1. 4 equals 1.0 mg of IgG.
Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Anti-ASH2L Antibody (A295266) was used for IP at 6 µg per reaction. ASH2L was also immunoprecipitated with another rabbit anti-ASH2L antibody. For blotting immunoprecipitated ASH2L, the other antibody was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Samples: Whole cell lysate (50 µg) from HEK293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Anti-ASH2L Antibody (A295266) was used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Publishing research using Anti-ASH2L Antibody (A295266)? Please let us know so that we can list the citation on this page.
Alternative products to Anti-ASH2L Antibody (A295266)
![Western Blot - Anti-ASH2L Antibody [ARC0326] (A305306) - Antibodies.com](https://cdn.antibodies.com/image/catalog/305/A305306_1.jpg?profile=product_alternative)
