Anti-APE1 Antibody (A83661)

Name: Anti-APE1 Antibody
Brand: Antibodies.com
SKU: A83661
Price: 495.0 USD
Availability: InStock

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$495

Goat polyclonal antibody to APE1 for ELISA, WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 4-6 business days.

Specifications

Name

Anti-APE1 Antibody

Description

Goat polyclonal antibody to APE1.

Applications

ELISA, WB, IHC

Dilutions

ELISA: 1:16,000, WB: 0.1-0.3µg/ml, IHC: 4-6µg/ml

Reactivity

Human

Immunogen

Synthetic peptide corresponding to the N terminal region of human APE1.

Sequence

PKRGKKGAVAEDGD-C

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Molecular Weight

37 kDa

Predicted MW

35.6kDa

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

AP endonuclease 1, APE, APE-1, APEN, APEX, APEX nuclease, APEX1, Apurinic-apyrimidinic endonuclease 1, APX, DNA repair nuclease/redox regulator APEX1, DNA-(apurinic or apyrimidinic site) endonuclease, HAP1, Redox factor-1, REF-1, REF1

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Western Blot - Anti-APE1 Antibody (A83661)

APE1 expression in A431 (A), HeLa (B), and MCF7 (C) nuclear lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed for 1 hour with Anti-APE1 Antibody (A83661) at 0.3µg/ml and detected by chemiluminescence.

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Immunohistochemistry - Anti-APE1 Antibody (A83661)

APE1 expression in Human Breast analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in Tris/EDTA buffer, pH 9. Staining was performed with Anti-APE1 Antibody (A83661) at 4µg/ml and revealed with horseradish peroxidase (HRP).

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