Anti-APAF1 Antibody (A88251)

Name: Anti-APAF1 Antibody
Brand: Antibodies.com
SKU: A88251
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to APAF1 for WB and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-APAF1 Antibody

Description

Rabbit polyclonal antibody to APAF1.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 470-600 of human APAF1 (NP_863651.1).

Sequence

PHTLSPDQEDCMYWYNFLAYHMASAKMHKELCALMFSLDWIKAKTELVGPAHLIHEFVEYRHILDEKDCAVSENFQEFLSLNGHLLGRQPFPNIVQLGLCEPETSEVYQQAKLQAKQEVDNGMLYLEWINK

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

150 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

APAF-1, Apoptotic protease-activating factor 1, KIAA0413

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (6)

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Western Blot - Anti-APAF1 Antibody (A88251)

Western blot analysis of extracts of Mouse testis, using Anti-APAF1 Antibody (A88251) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Western Blot - Anti-APAF1 Antibody (A88251)

Western blot analysis of extracts of various cell lines, using Anti-APAF1 Antibody (A88251) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 30s.

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Immunofluorescence - Anti-APAF1 Antibody (A88251)

Immunofluorescence analysis of A-549 cells using Anti-APAF1 Antibody (A88251) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Immunofluorescence - Anti-APAF1 Antibody (A88251)

Immunofluorescence analysis of HeLa cells using Anti-APAF1 Antibody (A88251) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).