Anti-Annexin A1 Antibody [ANXA1/3566] - BSA and Azide free
Description
Mouse monoclonal [ANXA1/3566] antibody to Annexin A1.
Specificity
The ANXA1 gene belongs to the annexin family, and contains 4 annexin repeats. A pair of annexin repeats may form one binding site for calcium and a phospholipid. ANXA1 promotes membrane fusion and is involved in exocytosis. The gene for ANXA1 is upregulated in hairy cell leukemia (HCL), and its protein expression is specific for HCL. Detection of ANXA1 provides a simple, highly sensitive and specific assay for diagnosing HCL. Annexin A1 has also been found to be protective against DNA damage induced by heat in breast cancer cells, suggesting it is involved in tumor suppressive and protective activities, and also is associated with treatment resistance.
SDS-PAGE analysis of Anti-Annexin A1 Antibody [ANXA1/3566] under non-reduced and reduced conditions; showing intact IgG and intact heavy and light chains, respectively. SDS-PAGE analysis confirms the integrity and purity of the antibody.
Immunofluorescent analysis of paraformaldehyde fixed HeLa cells stained with Anti-Annexin A1 Antibody [ANXA1/3566] followed by Goat Anti-Mouse IgG (CF® 488) (Green). Nuclear counterstain is RedDot.
Flow cytometric analysis of PFA fixed HeLa cells using Anti-Annexin A1 Antibody [ANXA1/3566] followed by Goat Anti-Mouse IgG (CF® 488) (Blue). Isotype Control (Red).
Anti-Annexin A1 Antibody [ANXA1/3566] - BSA and Azide free (A251997)
Analysis of protein array containing more than 19,000 full-length human proteins using Anti-Annexin A1 Antibody [ANXA1/3566]. Z-Score and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target; a MAb is considered to be specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
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