Recombinant human monoclonal antibody to Mesothelin.
Applications
ELISA, FACS, Functional Assay, In Vivo
Reactivity
Human, Mouse
Host
Human
Clonality
Monoclonal
Isotype
IgG1
Light Chains
lambda
Conjugate
Unconjugated
Purification
Protein A affinity chromatography.
Concentration
Reconstitution dependent.
Molecular Weight
This antibody has a predicted MW of 143.8 kDa.
Purity
> 95% (by SDS-PAGE and SEC-HPLC).
Product Form
Lyophilized
Reconstitution
Reconstitute with 100µl of sterile double-distilled water to bring antibody to 1mg/ml concentration. Gently shake to solubilize completely. Do not vortex!
Formulation
Lyophilized from 25mM Histadine, pH 6.2, with 8% Sucrose and 0.01% Tween80.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
SDS-PAGE analysis of Anetumab Biosimilar - Anti-Mesothelin Antibody - Low endotoxin, Azide free (A323282) under reducing (R) conditions confirms that the purity of this antibody is greater than 95%.
SEC-HPLC analysis of Anetumab Biosimilar - Anti-Mesothelin Antibody - Low endotoxin, Azide free (A323282) confirms that the purity of this antibody is 98.58%.
ASPC-1 cells were stained with Anetumab Biosimilar - Anti-Mesothelin Antibody - Low endotoxin, Azide free (A323282) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC50 = 0.1822 µg/ml.
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