Rabbit polyclonal antibody to Androgen Receptor (Phospho-Tyr363)
Specificity
The antibody detects endogenous levels of Androgen Receptor only when phosphorylated at tyrosine 363.
Applications
WB
Dilutions
Western blotting: 1:500~1:1000
Reactivity
Human, Mouse
Immunogen
Peptide sequence around phosphorylation site of tyrosine 363 (D-Y-Y(p)-N-F) derived from Human Androgen Receptor.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20˚C / 1 year
Synonyms
AR, DHTR, Dihydrotestosterone receptor, NR3C4, Nuclear receptor subfamily 3 group C member 4
Western blot analysis of extracts from HuvEc cells (Lane 2), COS7 cells (Lane 3) and JK cells (Lane 4), using Androgen Receptor (Phospho-Tyr363) Antibody #11761. The lane on the left is treated with antigen-specific peptide.
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