Anti-AMPK alpha 1 (phospho Ser485) Antibody (A91485)

Name: Anti-AMPK alpha 1 (phospho Ser485) Antibody
Brand: Antibodies.com
SKU: A91485
Price: 485.0 USD
Availability: InStock

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$485

Rabbit polyclonal antibody to AMPK alpha 1 (phospho Ser485) for WB.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-AMPK alpha 1 (phospho Ser485) Antibody

Description

Rabbit polyclonal antibody to AMPK alpha 1 (phospho Ser485).

Applications

Dilutions

WB: 1:500-1:2,000

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around S485 of human PRKAA1 (NP_006242.5).

Sequence

SGSVS

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

56 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

5'-AMP-activated protein kinase catalytic subunit alpha-1, ACACA kinase, Acetyl-CoA carboxylase kinase, AMPK subunit alpha-1, AMPK1, EC:2.7.11.26, EC:2.7.11.31, HMGCR kinase, Hydroxymethylglutaryl-CoA reductase kinase, PRKAA1, Tau-protein kinase PRKAA1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (1)

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Western Blot - Anti-AMPK alpha 1 (phospho Ser485) Antibody (A91485)

Western blot analysis of extracts of 293T cells, using Anti-AMPK alpha 1 (phospho Ser485) Antibody (A91485) at 1:1,000 dilution. 293T cells were treated by oligomycin (0. 5 uM) at 37°C for 30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 1s.

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