AMF expression in Human Heart lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-AMF Antibody (A84737) at 0.3µg/ml and detected by chemiluminescence.
AMF expression in HeLa (A), A431 (B), A549 (C), MCF7 (D), Jurkat (E), and K562 (F) lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-AMF Antibody (A84737) at 0.05µg/ml and detected by chemiluminescence.
AMF expression in Human Testis analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-AMF Antibody (A84737) at 3.75µg/ml and revealed with alkaline phosphatase (AP).
AMF expression in Human Thyroid analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-AMF Antibody (A84737) at 3.75µg/ml and revealed with alkaline phosphatase (AP).
AMF expression in Human Kidney analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-AMF Antibody (A84737) at 3.75µg/ml and revealed with alkaline phosphatase (AP).
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