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Anti-alpha Tubulin Antibody (A80425)

Western Blot - Anti-alpha Tubulin Antibody (A80425) - Antibodies.com Immunohistochemistry - Anti-alpha Tubulin Antibody (A80425) - Antibodies.com Immunohistochemistry - Anti-alpha Tubulin Antibody (A80425) - Antibodies.com Immunohistochemistry - Anti-alpha Tubulin Antibody (A80425) - Antibodies.com Immunofluorescence - Anti-alpha Tubulin Antibody (A80425) - Antibodies.com Immunofluorescence - Anti-alpha Tubulin Antibody (A80425) - Antibodies.com Immunofluorescence - Anti-alpha Tubulin Antibody (A80425) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to alpha Tubulin for WB, IHC and ICC/IF.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 5-8 business days.

  • Specifications

Name
Anti-alpha Tubulin Antibody
Description
Rabbit polyclonal antibody to alpha Tubulin.
Applications
WB, IHC, ICC/IF
Dilutions
WB: 1:500-1:2,000, IHC: 1:25-1:50, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human a-Tubulin (P68366).
Sequence
MRECISVHVGQAGVQMGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFTTFFCETGAGKHVPRAVFVDLEPTVIDEIRNGPYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDPVLDRIRKLSDQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTA
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
55 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Alpha-tubulin 1, Alpha-tubulin 3, Alpha-tubulin 3E, Testis-specific alpha-tubulin, TUBA1, TUBA1A, TUBA3, TUBA3E, TUBA4A, Tubulin alpha-1 chain, Tubulin alpha-1A chain, Tubulin alpha-3 chain, Tubulin alpha-3E chain, Tubulin alpha-4A chain, Tubulin B-alpha-1, Tubulin H2-alpha
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (7)

Western Blot - Anti-alpha Tubulin Antibody (A80425) - Antibodies.comEnlarge Image

Western Blot - Anti-alpha Tubulin Antibody (A80425)

Western blot analysis of extracts of various cell lines, using Anti-alpha Tubulin Antibody (A80425) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit.
Immunohistochemistry - Anti-alpha Tubulin Antibody (A80425) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-alpha Tubulin Antibody (A80425)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-alpha Tubulin Antibody (A80425)Immunohistochemistry analysis of paraffin-embedded rat brain using alpha-Tubulin Antibody (AC014) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-alpha Tubulin Antibody (A80425) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-alpha Tubulin Antibody (A80425)

Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-alpha Tubulin Antibody (A80425)Immunohistochemistry analysis of paraffin-embedded rat kidney using alpha-Tubulin Antibody (AC014) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-alpha Tubulin Antibody (A80425) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-alpha Tubulin Antibody (A80425)

Immunohistochemistry analysis of paraffin-embedded rat heart using Anti-alpha Tubulin Antibody (A80425)Immunohistochemistry analysis of paraffin-embedded rat heart using alpha-Tubulin Antibody (AC014) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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