Anti-alpha Tubulin Antibody (A17304)

Western blot analysis of extracts of various cell lines, using Anti-alpha Tubulin Antibody (A17304) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

Western blot analysis of various lysates, using Anti-alpha Tubulin Antibody (A17304) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

Immunofluorescence analysis of C6 cells using Anti-alpha Tubulin Antibody (A17304) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

Immunofluorescence analysis of NIH-3T3 cells using Anti-alpha Tubulin Antibody (A17304) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).