Rabbit polyclonal antibody to alpha Synuclein (phospho Ser129).
Specificity
This antibody binds to phosphorylated serine 129 on alpha synuclein. Does not detect unphosphorylated serine 129 alpha synuclein. Bands above 15 kDa are oligomers.
Applications
WB, ELISA, ICC/IF, IHC
Dilutions
WB: 1:1,000, ICC/IF: 1:500
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide of Human Alpha Synuclein pSer129 (40-140 aa), conjugated to Keyhole Limpet Haemocyanin (KLH)
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Peptide affinity purification.
Concentration
500 µg/ml
Molecular Weight
15 kDa / 45 kDa / 75 kDa / 100 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Alpha-synuclein, NACP, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, PARK1, SNCA
Immunocytochemistry/Immunofluorescence analysis of primary mouse hippocampal neurons treated with 100 nM sonicated mouse alpha synuclein PFFs, using Anti-alpha Synuclein (phospho Ser129) Antibody (A305044). Phosphorylated alpha synuclein was visible in perinucleus and neurites(A) compared to untreated control (B).
Immunohistochemistry analysis of human brain, fixed in formalin and paraffin-embedded. The Primary Antibody used was Anti-alpha Synuclein (phospho Ser129) Antibody (A305044) at 1:50 for 30 minutes at room temperature. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.
Western Blot - Anti-alpha Synuclein (phospho Ser129) Antibody (A305044)
Western blot analysis of Human, mouse brain lysate showing detection of ~16 kDa Alpha Synuclein pSer129 protein using Anti-alpha Synuclein (phospho Ser129) Antibody (A305044) at 1:1,000 for 2 hours at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: human brain lysate. Lane 3: mouse brain lysate. Lane 4: human Alpha Synuclein Monomer (0.5 µg). Load: 15 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at room temperature. Color Development: ECL solution for 5 minutes at room temperature. Predicted/Observed Size: ~16 kDa. Other Band(s): 100, 75, 45, 30,16 kDa.
Immunocytochemistry/Immunofluorescence analysis of rat primary hippocampal neurons treated with active alpha synuclein protein aggregate (SPR-322) at 4 µg/ml to induce fibrils, fixed in 4% paraformaldehyde, using Anti-alpha Synuclein (phospho Ser129) Antibody (A305044), at 1:500 for 24 hours at 4°C. The secondary antibody used was Goat Anti-Rabbit Alexa Fluor 488 at 1:700 for 1 hour at room temperature. Counterstain: Guinea Pig Anti-NeuN (red) neuronal marker (Donkey Anti-Guinea Pig Alexa Fluor 647 1:700); Hoechst (blue) nuclear stain at 1:6000, 1:3000 for 60 minutes at room temperature, 5 minutes at room temperature. Magnification: 20X.
Immunohistochemistry analysis of mouse free floating brain sections, fixed in PFA. The Primary Antibody used was Anti-alpha Synuclein (phospho Ser129) Antibody (A305044) at 1:500 for overnight at 4°C with gentle agitation. Counterstain: Hoechst. Magnification: 63X. A) Right hemisphere (striatum) injected with alpha synuclein AAV vector. B) Control. Alpha synuclein streaks are visible at injection site, but not control, 4 months after injection.
![Immunocytochemistry/Immunofluorescence - Anti-alpha Synuclein (phospho Ser129) Antibody [J18] (A304933) - Antibodies.com](https://cdn.antibodies.com/image/catalog/304/A304933_1.png?profile=product_alternative)
![Immunocytochemistry/Immunofluorescence - Anti-alpha Synuclein Antibody [4F1] (A304962) - Antibodies.com](https://cdn.antibodies.com/image/catalog/304/A304962_1.png?profile=product_alternative)
![Western Blot - Anti-alpha Synuclein Antibody [3C11] (A304959) - Antibodies.com](https://cdn.antibodies.com/image/catalog/304/A304959_1.png?profile=product_alternative)
![Immunocytochemistry/Immunofluorescence - Anti-alpha Synuclein Antibody [3F8] (A304961) - Antibodies.com](https://cdn.antibodies.com/image/catalog/304/A304961_1.png?profile=product_alternative)
![Western Blot - Anti-alpha Synuclein Antibody [10H7] (A304960) - Antibodies.com](https://cdn.antibodies.com/image/catalog/304/A304960_1.png?profile=product_alternative)
