Anti-alpha Synuclein Antibody (A85289)

Immunofluorescent analysis of a section of rat cerebellum stained with Anti-Alpha-synuclein Antibody (A85289), dilution 1:3,000, in red, and co-stained with Anti-GFAP Antibody (A85419), dilution 1:5,000, in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of the rat with 4% paraformaldehyde, the brain was post fixed for 24 hours, cut to 45 µm, and free-floating sections were stained with above antibodies. The alpha-synuclein protein is concentrated in presynaptic regions in the granular and molecular layers, while the GFAP antibody stains the network of Bergmann and astroglial cells.

Western blot analysis of different tissue lysates using Anti-Alpha-synuclein Antibody (A85289), dilution 1:2,000 in green. The lanes contain: [Lane 1] protein standard (red), [Lane 2] rat brain, [Lane 3] rat spinal cord, [Lane 4] mouse brain, and [Lane 5] mouse spinal cord. The strong band at about 15 kDa corresponds to the alpha-synuclein protein in brain extracts, which are rich in synapses, while a weaker band is seen in spinal cord extracts where synapses are a more minor component.

Mixed rat neuron-glial cultures stained with Anti-Alpha-Synuclein Antibody (red) and Anti-MAP2 Antibody (green). The alpha-synuclein antibody stains vesicular structures - the perikarya and processes of the neurons in this image. Note that some of the neuronal perikarya contain much more alpha-synuclein than others. The blue channel shows the localization of DNA.

Blot of Anti-Alpha-Synuclein Antibody on crude extract of rat brain, showing strong band at 15 kDa.