Anti-alpha Synuclein Antibody (A304963)

Western blot analysis of human Truncated Alpha Synuclein Protein showing detection of Alpha Synuclein protein using Anti-alpha Synuclein Antibody (A304963) at 1:1,000 for 2 hours at room temperature with shaking. Lane 1: MW Ladder. Lane 2: hASYN aa 1-114 (5 uL). Load: 5 uL. Block: 5% Skim Milk Powder in TBST. The secondary antibody used was Goat anti-rabbit IgG:HRP at 1:4,000 for 1 hour at room temperature with shaking.

Western blot analysis of mouse brain showing detection of ~14.46kDa Alpha Synuclein protein using Anti-alpha Synuclein Antibody (A304963) at 1:1,000 for 2 hours at room temperature with shaking. Lane 1: Molecular Weight Ladder (MW). Lane 2: mouse Brain. Load: 15 µg. Block: 5% Skim Milk powder in TBST. The secondary antibody used was Goat anti-rabbit IgG:HRP at 1:4,000 for 1 hour at room temperature with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 minutes in room temperature. Predicted/Observed Size: ~14.46kDa. Other Band(s): Higher molecular weight oligomers.

Western blot analysis of human Recombinant Protein showing detection of Alpha Synuclein protein using Anti-alpha Synuclein Antibody (A304963) at 1:1,000 for 2 hours at room temperature with shaking. Lane 1: MW ladder. Lane 2: Alpha Synuclein. Lane 3: Beta Synuclein. Lane 4: Gamma Synuclein. Load: 0.5 ug. Block: 5% Skim Milk powder in TBST. The secondary antibody used was Goat anti-rabbit IgG:HRP at 1:4,000 for 1 hour at room temperature with shaking. Alpha synuclein antibody shows cross-reactivity with beta synuclein but not gamma synuclein..

Immunohistochemistry analysis of human cerebellum, fixed in formalin and paraffin-embedded. The Primary Antibody used was Anti-alpha Synuclein Antibody (A304963).