Anti-alpha Synuclein Antibody [2A7] (A85290)

Immunofluorescent analysis of rat olfactory bulb section stained with Anti-MeCP2 Antibody (A104322), at a dilution of 1:2,000, in red, and co-stained with Anti-alpha Synuclein Antibody [2A7] (A85290), at a dilution of 1:1,000, in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45µM, and free-floating sections were stained with the above antibodies. Anti-MeCP2 Antibody (A104322) specifically labels the nuclei of neuronal cells while Anti-alpha Synuclein Antibody [2A7] (A85290) reveals alpha Synuclein protein concentrated in presynaptic regions.

Dot blot analysis of various truncated and mutant construct applied to PVDF membranes in about of 400ng of each protein. These were the first 60 amino acids of Anti-alpha Synuclein Antibody [2A7] (A85290), amino acids 61 to 140 (61-140), full length but incorporating the A30P and A53T mutations seen associated with familial forms of Parkinson’s disease (A30P/A53T), full length but with the central NAC region of amino acids 61-95 missing (d61-95) and full length alpha synuclein. The epitopes for Anti-alpha Synuclein Antibody [2A7] (A85290) are clearly in the NAC region from 61-95 within amino acids 61-140, but do not appear to bind amino acids 1-60, suggesting that this region has low immunogenicity.

Immunofluorescent analysis of rat hippocampus section costained with Anti-alpha Synuclein Antibody [2A7] (A85290) at a dilution of 1:1,000 (green) and Anti-MeCP2 Antibody (A104322) at a dilution of 1:2,000 (red). Nuclei were stained with DAPI (blue). Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 1 hour, cut to 45 µM, and free-floating sections were stained with above antibodies. The a-synuclein protein is concentrated in synaptic regions, and the MeCP2 antibody stains the nuclei of neuronal cells.

Immunofluorescent analysis of rat olfactory bulb section costained with Anti-alpha Synuclein Antibody [2A7] (A85290) at a dilution of 1:1,000 (red) and Anti-GFAP Antibody (A85419) at a dilution of 1:5,000 (green). Nuclei were stained with DAPI (blue). Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45µM, and free floating sections were stained with above antibodies. The a-synuclein protein is concentrated in synaptic regions, while the GFAP antibody stains the filamentous backbone of astroglial cells.

Immunohistochemistry analysis of a NBF fixed paraffin embedded human cerebellum section with Anti-alpha Synuclein Antibody [2A7] (A85290) at a dilution of 1:1,000 detected in DAB (brown) using the Vector Labs ImmPRESS method and reagents with citra buffer retrieval. Counterstained with Hematoxylin (blue). The a-synuclein antibody synaptic regions in the granular and molecular layers of the cerebellum. Note: this antibody performs well in testing with both 4% PFA and standard NBF fixed tissues.

Immunohistochemistry analysis of the cortex of a patient with Parkinson’s disease (PD) stained with Anti-alpha Synuclein Antibody [2A7] (A85290) at a dilution of 1:1,000 with horse radish peroxidase and DAB. The Lewy bodies and other typical inclusions of PD are seen in brown.

Western blot (left) and Ponceau S stained blot (right) analysis of [Lane 1] protein standard, recombinant full length human [Lane 2] alpha, [Lane 3] beta, and [Lane 4] gamma Synuclein in lanes 2, 3 and 4 respectively. Anti-alpha Synuclein Antibody [2A7] (A85290) reacts strongly with alpha Synuclein and shows no reaction with the other proteins.