Anti-alpha Synuclein Antibody [2A7] (A85290)

Immunofluorescent analysis of rat cerebellum section co-stained with Anti-a-Synuclein Antibody (1:1,000 | red) and Anti-GFAP Antibody (A85419 | 1:5,000 | green). The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45µM, and free-floating sections were stained with the above antibodies. The a-synuclein protein is concentrated in synaptic regions, while the Anti-GFAP Antibody stains the filamentous cytoskeleton of Bergmann glia and astrocytic cells.

Western blot analysis of tissue lysates using Anti-a-Synuclein Antibody (1:1,000 | green): [1] protein standard in red, [2] whole rat brain lysate, [3] rat spinal cord lysate. Strong band at about 15kDa corresponds to a-synuclein protein.

Mixed neuron-glial cultures stained with Anti-a-Synuclein Antibody (red) and Anti-MAP2 Antibody (A85363 | green). The Anti-a-Synuclein Antibody stains vesicular structures - the perikarya and processes of the neurons in this image. Note that some of the neuronal perikarya contain much more alpha-synuclein than others. The blue channel shows the localization of DNA.

Blots of Anti-a-Synuclein Antibody on recombinant alpha-synuclein (left lane) and crude extract of mouse brain (right lane).