Anti-alpha Actinin 4 Antibody [ARC1959] (A307957)

Name: Anti-alpha Actinin 4 Antibody [ARC1959]
Brand: Antibodies.com
SKU: A307957
Price: 485.0 USD
Availability: InStock

Enlarge Image

$485

Rabbit monoclonal [ARC1959] antibody to alpha Actinin 4 for WB, ICC/IF and IP.

100% Guarantee

Price Match Guarantee

Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-alpha Actinin 4 Antibody [ARC1959]

Description

Rabbit monoclonal [ARC1959] antibody to alpha Actinin 4.

Applications

WB, ICC/IF, IP

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human a-Actinin-4 (O43707).

Sequence

MVDYHAANQSYQYGPSSAGNGAGGGGSMGDYMAQEDDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIDEDFRDGLKLMLLLEVISGERLPKPE

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1959

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

100 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

ACTN4, Alpha-actinin-4, Non-muscle alpha-actinin 4

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

Enlarge Image

Western Blot - Anti-alpha Actinin 4 Antibody [ARC1959] (A307957)

Western blot analysis of extracts of various cell lines, using Anti-alpha Actinin 4 Antibody [ARC1959] (A307957) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

Enlarge Image

Immunofluorescence - Anti-alpha Actinin 4 Antibody [ARC1959] (A307957)

Immunofluorescence analysis of C6 cells using Anti-alpha Actinin 4 Antibody [ARC1959] (A307957) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

Enlarge Image

Immunofluorescence - Anti-alpha Actinin 4 Antibody [ARC1959] (A307957)

Immunofluorescence analysis of NIH-3T3 cells using Anti-alpha Actinin 4 Antibody [ARC1959] (A307957) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

Enlarge Image

Immunofluorescence - Anti-alpha Actinin 4 Antibody [ARC1959] (A307957)

Immunofluorescence analysis of U-2 OS cells using Anti-alpha Actinin 4 Antibody [ARC1959] (A307957) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).