Rabbit polyclonal antibody to alpha 1 Antitrypsin.
Applications
WB, ICC/IF
Dilutions
WB: 1:500-1:2,000, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 25-315 of human Alpha-1 Antitrypsin (Alpha-1 Antitrypsin (SERPINA1)) (NP_000286.3).
Western Blot - Anti-alpha 1 Antitrypsin Antibody (A87574)
Western blot analysis of extracts of various cell lines, using Anti-alpha 1 Antitrypsin Antibody (A87574) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST.
Immunofluorescence analysis of PC-12 cells using Anti-alpha 1 Antitrypsin Antibody (A87574) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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