Anti-ALIX Antibody [3A9] (A279284)

Anti-ALIX Antibody [3A9]

Mouse monoclonal [3A9] antibody to ALIX.

This antibody recognises the apoptosis-linked gene-2 interacting protein X (ALIX), also known as Programmed cell death 6-interacting protein or Hp95. ALIX is a 868 amino acid ~90kDa cytoplasmic protein containing a single BRO1 domain implicated in endosomal targeting. ALIX was first reported to play a role in apoptosis dependent on its association with PDCD6 but has subsequently shown to be involved in endocytosis , viral budding and actin cytoskeletal assembly. Mouse anti Alix, clone 3A9 has been used extensively for the detection of ALIX by Western blotting and for the identification of an ALIX homologue in the sea urchin Paracentrotus lividus.

WB, IHC-P, IP, ELISA, IF

This product does not require protein digestion pre-treatment of paraffin sections.

Human, Xenopus, Rat, Mouse

Alix-GST fusion protein.

Mouse

Monoclonal

3A9

IgG1

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti ALIX antibody, clone 3A9 recognizes the apoptosis-linked gene-2 interacting protein X (ALIX), also known as Programmed cell death 6-interacting protein or Hp95. ALIX is a 868 amino acid ~90kDa cytoplasmic protein containing a single BRO1 domain implicated in endosomal targeting (Kim et al. 2005). ALIX was first reported to play a role in apoptosis dependent on its association with PDCD6 (Mahul-Mellier et al. 2006) but has subsequently shown to be involved in endocytosis (Matsuo et al. 2004), viral budding (Irie et al. 2008) and actin cytoskeletal assembly (Pan et al. 2006).Mouse anti Alix, clone 3A9 has been used extensively for the detection of ALIX by Western blotting (Sun et al. 2016) and for the identification of an ALIX homologue in the sea urchin Paracentrotus lividus (Romancino et al. 2013).

AIP1, ALG-2-interacting protein 1, ALG-2-interacting protein X, Hp95, KIAA1375, PDCD6-interacting protein, PDCD6IP, Programmed cell death 6-interacting protein