Anti-Aldose reductase Antibody (A13594)

Name: Anti-Aldose reductase Antibody
Brand: Antibodies.com
SKU: A13594
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to Aldose reductase for WB and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-Aldose reductase Antibody

Description

Rabbit polyclonal antibody to Aldose reductase.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-316 of human AKR1B1 (NP_001619.1).

Sequence

MASRLLLNNGAKMPILGLGTWKSPPGQVTEAVKVAIDVGYRHIDCAHVYQNENEVGVAIQEKLREQVVKREELFIVSKLWCTYHEKGLVKGACQKTLSDLKLDYLDLYLIHWPTGFKPGKEFFPLDESGNVVPSDTNILDTWAAMEELVDEGLVKAIGISNFNHLQVEMILNKPGLKYKPAVNQIECHPYLTQEKLIQYCQSKGIVVTAYSPLGSPDRPWAKPEDPSLLEDPRIKAIAAKHNKTTAQVLIRFPMQRNLVVIPKSVTPERIAENFKVFDFELSSQDMTTLLSYNRNWRVCALLSCTSHKDYPFHEEF

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

36 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

AKR1B10, AKR1B11, Aldehyde reductase, Aldo-keto reductase family 1 member B1, Aldo-keto reductase family 1 member B10, Aldose reductase-like, Aldose reductase-related protein, ALDR1, ALR2, AR, ARL-1, ARP, EC:1.1.1.21, hARP, SI reductase, Small intestine reductase

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

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Western Blot - Anti-Aldose reductase Antibody (A13594)

Western blot analysis of extracts of various cell lines, using Anti-Aldose reductase Antibody (A13594) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Immunofluorescence - Anti-Aldose reductase Antibody (A13594)

Immunofluorescence analysis of HeLa cells using Anti-Aldose reductase Antibody (A13594) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Immunofluorescence - Anti-Aldose reductase Antibody (A13594)

Immunofluorescence analysis of HepG2 cells using Anti-Aldose reductase Antibody (A13594) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Immunofluorescence - Anti-Aldose reductase Antibody (A13594)

Immunofluorescence analysis of NIH/3T3 cells using Anti-Aldose reductase Antibody (A13594) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).