Anti-ALDH1L1 Antibody [4A12] (A85315)

Immunofluorescent analysis of mixed cortical neuron-glial cell culture from an E20 rat stained with Anti-ALDH1L1 Antibody [4A12] (A85315), dilution 1:1,000, in green, and co-stained with Anti-Vimentin Antibody (A85421), dilution 1:5,000, in red. The blue is DAPI staining of nuclear DNA. Anti-ALDH1L1 Antibody [4A12] produces strong cytoplasmic staining of astrocytic glial cells, while the Anti-Vimentin Antibody labels the intermediate filament cytoskeleton in fibroblasts and other glial cells. Some cells, presumably developing asrocytes, are stained by both ALDH1L1 and vimentin and so appear yellow.

Western blot analysis of rat and mouse tissue lysates using Anti-ALDH1L1 Antibody [4A12] (A85315), dilution 1:5,000, in green. The lanes contain: [Lane 1] protein standard (red), rat tissue lysates: [Lane 2] heart, [Lane 3] liver, [Lane 4] kidney, [Lane 5] lung, [Lane 6] brain, and [Lane 7] spinal cord; and mouse tissue lysates: [Lane 8] brain, and [Lane 9] spinal cord. The band at 100 kDa corresponds to ALDH1L1 protein. In line with published work, ALDH1L1 is a major protein of liver and CNS tissue.

Neuron-glia cell mixture cultures stained with Anti-Aldehyde Dehydrogenase H1L1 Antibody (green) and Anti-GFAP Antibody (A85419 | red). Blue is a DNA stain. Anti-Aldehyde Dehydrogenase H1L1 Antibody stains astrocytes and is excluded from neuron cells. Anti-Aldehyde Dehydrogenase H1L1 Antibody stains the astrocytes cell body and processes, whereas Anti-GFAP Antibody labels the intermediate filament cytoskeleton in a subset of astrocytes. Astrocytes stained by both Anti-ALDH1L1 Antibody and Anti-GFAP Antibody appear yellow.

Blot of rat liver tissue homogenates blotted with Anti-Aldehyde Dehydrogenase H1L1 Antibody. Anti-Aldehyde Dehydrogenase H1L1 Antibody binding produces a strong band at ~100 kDa.